SRP6369
AKR1D1 human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
3o5bred, Aldo-keto reductase family 1, CBAS2, SRD5B1, member D1
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About This Item
UNSPSC Code:
12352200
biological source
human
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
liquid
mol wt
39.5 kDa (346 aa, 1-326 aa + His Tag)
packaging
pkg of 100 μg
concentration
0.5 mg/mL
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... AKR1D1(6718)
General description
AKR1D1 (aldo-keto reductase 1D1) is the only steroid 5β-reductase in humans. This enzyme shows major expression in liver and resides in cytoplasm. It is a member of the unique subfamily, AKR1D, of the aldo-keto reductase (AKR) superfamily, that are NAD(P)(H)-dependent oxidoreductases. AKR1D1 contains the characteristic (α/β)8 triosephosphate isomerase barrel in its core region, and has a molecular weight of 37kDa. Its C-terminal contains the active site residing on the β strands, and the ligand binding site is formed by long flexible loops.
Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biochem/physiol Actions
AKR1D1 (aldo-keto reductase 1D1) catalyzes the reduction of Δ4-3-ketosteroids to 5β-dihydrosteroids in a stereospecific manner, and is responsible for inducing A/B cis-configuration of bile acids. It is a critical enzyme for steroid hormone metabolism (except estrogens) and bile acid biosynthesis. Mutations in this gene are linked with bile acid deficiency resulting in usually fatal neonatal hepatitis and cholestasis. Variants in this gene influence the expression of p450 enzymes, such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, and CYP2B6, where up-regulation of AKR1D1 results in overexpression of these enzymes. Hence, this enzyme might indirectly be responsible for variation in drug metabolism or efficacy and adverse drug events in different individuals.
Physical form
0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 100 mM NaCl and 20% glycerol.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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The rate-determining steps of aldo-keto reductases (AKRs), a study on human steroid 5?-reductase (AKR1D1).
Chen M et al
Chemico-Biological Interactions, 234, 360-365 (2015)
In-Depth Dissection of the P133R Mutation in Steroid 5?-Reductase (AKR1D1): A Molecular Basis of Bile Acid Deficiency.
Chen M et al
Biochemistry, 54(41), 6343-6351 (2015)
Genetic variation in aldo-keto reductase 1D1 (AKR1D1) affects the expression and activity of multiple cytochrome P450s.
Chaudhry AS et al
Drug Metabolism and Disposition, 41(8), 1538-1547 (2013)
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