SRP6369
AKR1D1 human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
3o5bred, Aldo-keto reductase family 1, CBAS2, SRD5B1, member D1
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About This Item
UNSPSC Code:
12352200
biological source
human
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
liquid
mol wt
39.5 kDa (346 aa, 1-326 aa + His Tag)
packaging
pkg of 100 μg
concentration
0.5 mg/mL
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... AKR1D1(6718)
General description
AKR1D1 (aldo-keto reductase 1D1) is the only steroid 5β-reductase in humans. This enzyme shows major expression in liver and resides in cytoplasm.[1] It is a member of the unique subfamily, AKR1D, of the aldo-keto reductase (AKR) superfamily, that are NAD(P)(H)-dependent oxidoreductases. AKR1D1 contains the characteristic (α/β)8 triosephosphate isomerase barrel in its core region, and has a molecular weight of 37kDa. Its C-terminal contains the active site residing on the β strands, and the ligand binding site is formed by long flexible loops.[2]
Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biochem/physiol Actions
AKR1D1 (aldo-keto reductase 1D1) catalyzes the reduction of Δ4-3-ketosteroids to 5β-dihydrosteroids in a stereospecific manner, and is responsible for inducing A/B cis-configuration of bile acids. It is a critical enzyme for steroid hormone metabolism (except estrogens) and bile acid biosynthesis. Mutations in this gene are linked with bile acid deficiency resulting in usually fatal neonatal hepatitis and cholestasis.[1] Variants in this gene influence the expression of p450 enzymes, such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, and CYP2B6, where up-regulation of AKR1D1 results in overexpression of these enzymes. Hence, this enzyme might indirectly be responsible for variation in drug metabolism or efficacy and adverse drug events in different individuals.[3]
Physical form
0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 100 mM NaCl and 20% glycerol.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The rate-determining steps of aldo-keto reductases (AKRs), a study on human steroid 5?-reductase (AKR1D1).
Chen M et al
Chemico-Biological Interactions, 234, 360-365 (2015)
In-Depth Dissection of the P133R Mutation in Steroid 5?-Reductase (AKR1D1): A Molecular Basis of Bile Acid Deficiency.
Chen M et al
Biochemistry, 54(41), 6343-6351 (2015)
Genetic variation in aldo-keto reductase 1D1 (AKR1D1) affects the expression and activity of multiple cytochrome P450s.
Chaudhry AS et al
Drug Metabolism and Disposition, 41(8), 1538-1547 (2013)
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