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N3254

Sigma-Aldrich

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate, ≥97% (enzymatic), powder

Synonym(s):

4-Nitrophenyl phosphate bis(tris) salt

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About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
68189-42-4
Molecular Weight:
461.36
Beilstein/REAXYS Number:
1578142
EC Number:
269-198-6
MDL number:
MFCD00037009
UNSPSC Code:
12352204
PubChem Substance ID:
24897633
NACRES:
NA.83

product name

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

assay

≥97% (enzymatic)

form

powder

solubility

water: 50 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

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Application

4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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