Skip to Content
MilliporeSigma
All Photos(2)

Documents

M5528

Sigma-Aldrich

Methyl Blue

certified by the Biological Stain Commission, powder

Synonym(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
28983-56-4
Molecular Weight:
799.80
Colour Index Number:
42780
EC Number:
249-352-9
MDL number:
MFCD00058509
UNSPSC Code:
12171500
PubChem Substance ID:
329818015
NACRES:
NA.47

product name

Methyl Blue, certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

form

powder

solubility

H2O: 1 mg/mL, blue

εmax

≥700 at 594-610 nm in 5 mM HCl

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Suitability

Certified as aniline blue for use in Mallory′s connective tissue stain and as a substitute for fast green FCF in Gomori′s one-step trichrome procedure.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

>572.0 °F - Pensky-Martens closed cup

flash_point_c

> 300 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Reactive oxygen species and cellular interactions between Mycosphaerella fijiensis and banana.
Jesus M de, et al.
Tropical Plant Biology, 4, 134-134 (2011)
Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells.
Lee JW, et al.
Journal of Biomedical Materials Research Part A, 101, 1865-1865 (2013)
Juan Guo et al.
Journal of hazardous materials, 225-226, 63-73 (2012-05-23)
Fe nanoparticles@graphene composites (FGC) are successfully synthesized by using graphene oxide (GO) as a supporting matrix. GO is first treated with Fe(3+) to form Fe(3+)@GO complexes. Then, by adding NaBH(4) solution, Fe(3+) and GO are simultaneously reduced in situ to
Ja-An Annie Ho et al.
Analytical and bioanalytical chemistry, 391(2), 479-485 (2008-02-15)
Salmonellae are ubiquitous human pathogens, which pose a danger to the elderly and children. Due to the increased number of outbreaks of human illness associated with the consumption of contaminated products in the USA and many other countries, there is
A Arunarani et al.
Colloids and surfaces. B, Biointerfaces, 102, 379-384 (2012-09-27)
Basic Violet 3 and Acid Blue 93 are the most important group of synthetic colourants extensively used in textile industries for dyeing cotton, wool, silk and nylon. Release of these dye pollutants in to the environment adversely affects the human
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service