KEM0005
Lambda Exonuclease
Ultra-pure enzyme for nucleic acid modifications
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About This Item
Recommended Products
grade
for molecular biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
80,000 U/mg
concentration
5,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
Lambda Exonuclease is a highly processive 5′ → 3′ double-stranded exonuclease that degrades one strand of the duplex. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda exonuclease has greatly reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease will no initiate at a nick or gap.
Application
Suitable for applications requiring a highly processive 5′ – 3′ exonuclease which is active on both blunt and 5′ recessed ends.
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Components
Supplied with:KEM0005B (10X Lambda Exo Reaction Buffer)
Unit Definition
1 unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37° C.
Physical form
Supplied in 25 mM Tris-HCl, 50 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA and 50% glycerol at pH7.5 @ 25° C.
Other Notes
Source of protein: Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.
Unit size: 10,000 U
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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