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TGO

Sigma-Aldrich

ThermaGo™

increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming during PCR

Synonym(s):

Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart

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About This Item

UNSPSC Code:
12352200

form

lyophilized powder

packaging

units of 2500 units
units of 500 units
pkg of One unit of ThermaGo™ is defined as the amount required for maximum improvements in amplification reactions containing 1 unit of Taq DNA polymerase in a volume of 25 μl. 

technique(s)

PCR: suitable

color

opaque

shipped in

ambient

storage temp.

2-8°C

General description

ThermaGo™ is a double-stranded, modified nucleic acid that terminates the mispriming by interacting with DNA polymerase during the annealing and extension steps of PCR. It improves the specificity of Taq DNA polymerase. ThermaStop™ and ThermaGo act together during PCR amplification at different temperatures to increase specificity and prevent errors.

Application

ThermaGo™ is suitable for:
  • monoplex and multiplexed end-point single nucleotide polymorphism (SNP) genotyping
  • detection of low-copy target numbers during single-cell PCR and digital PCR
  • detection of low-copy target numbers in liquid biopsies/tumor samples
  • enhancing the accuracy of multiplex PCR for next-generation sequencing and other post PCR applications

Features and Benefits

  • Acts at higher temperatures during annealing and elongation steps
  • Improves the reproducibility
  • Detects low-copy number targets by suppressing non-specific targets
  • Minimizes signal scatter in digital & standard PCR
  • Minimizes mispriming and chimera production before next-generation sequencing (NSG)
  • Enhances end-point genotyping

Reconstitution

Dissolve in Tris pH 8.3 buffer

Legal Information

ThermaGo is a trademark of ThermaGenix Inc.
ThermaStop is a trademark of ThermaGenix Inc.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

ThermaStop™ PCR Additive

PCR additive added to master mix that stops nonspecific bands after cool down.

ThermaGenix Product Overview

How to stop nonspecific bands and off target primers in PCR.

ThermaStop™-RT PCR Additive

PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.

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