S3917
Single-strand Binding Protein from Escherichia coli
>95% (SDS-PAGE), for molecular biology, Single strand binding protein to enhance PCR specificity
Synonym(s):
SSB
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0.1 MG
$166.00
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0.1 MG
$166.00
0.5 MG
$573.00
About This Item
Recommended Products
grade
for molecular biology
Quality Level
assay
>95% (SDS-PAGE)
form
liquid
color
colorless
foreign activity
DNase and RNase, none detected
shipped in
wet ice
storage temp.
−20°C
General description
Application
Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
Biochem/physiol Actions
Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes.[1][5] Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard.[5] It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication.[5] SSBP mediates the recruitment of other proteins to their sites of function on DNA.[1]
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
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Find documentation for the products that you have recently purchased in the Document Library.
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.
Q Chou
Nucleic acids research, 20(16), 4371-4371 (1992-08-25)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 87, 5-5 (1989)
Structure of the SSB--DNA polymerase III interface and its role in DNA replication
Marceau AH, et al.
The Embo Journal, 30(20), 4236-4247 (2011)
J Kieleczawa et al.
Science (New York, N.Y.), 258(5089), 1787-1791 (1992-12-11)
When template DNA is saturated with a single-stranded DNA binding protein (SSB), strings of three or four contiguous hexanucleotides (hexamers) can cooperate through base-stacking interactions to prime DNA synthesis specifically from the 3' end of the string. Under the same
The Integron Integrase Efficiently Prevents the Melting Effect of Escherichia coli Single-Stranded DNA-Binding Protein on Folded attC Sites
Loot C, et al.
Journal of Bacteriology, 196(4), 762-771 (2014)
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