SRP6495

IGF 1R/CD221 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): IGF-1R, IGFIR, IGFR, IGFⅠR, Insulin-like Growth Factor 1 (IGF-1) Receptor, JTK13, MGC142170, MGC142172, MGC18216
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: β subunit calculated mol wt 23 kDa; A subunit calculated mol wt 80 kDa; single-chain calculated mol wt 104 kDa; 35 kDa; observed mol wt 120 kDa by SDS-PAGE (DTT-reduced.); observed mol wt 80 kDa
• packaging: pkg of 10 and 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P08069
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IGF1R(3480)

Description

General description

The Insulin-like Growth Factor 1 Receptor (IGF1R)is also known as CD221, JTK13, and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is made up of two α subunits and two β subunits. Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain. The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable