SRP0175

NSD2 catalytic domain Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonym(s): MMSET, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, REIIBP, Wolf-Hirschhorn syndrome candidate 1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• mol wt: 62 kDa
• packaging: pkg of 50 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_133330
• UniProt accession no.: O96028
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... WHSC1(7468)

Description

General description

NSD2 (nuclear SET domain-containing protein 20, or WHSC1 (Wolf-Hirschhorn syndrome candidate 1), is an oncogene belonging to the SET (su(var)3-9, enhancer-of-zeste and trithorax) domain containing NSD (nuclear receptor binding SET domain) protein family. It produces several different isoforms, with the predominant being MMSETII (multiple myeloma SET domain), which resides in the nucleus. Another common isoform is called REIIBP (interleukin-5 [IL-5] response element II binding protein), which is localized to cytoplasm and nucleus both. This gene also codes for highly expressed ACA11 (autoinhibited Ca²⁺-ATPase11), which is a small nucleolar RNA (snoRNA).[1] This gene is composed of 24 exons, spans 120kb, and codes for 1365 amino acid MMSETII.[2] NSD2 is a KMT (lysine methyltransferase) and its conserved SET domain is responsible for its catalytic capacity. This protein also contains multiple chromatin-associated domains containing four PHD (plant homeodomain) fingers, two PWWP (Pro-Trp-Trp-Pro) domains, and an HMG (high mobility group) box.[3]
Human NSD2 (MMSET) (GenBank Accession No. NM_133330), amino acids 941-1240 with N-terminal GST tag, MW = 62kDa, expressed in Escherichia coli.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

NSD2 (nuclear SET domain-containing protein 20, or WHSC1 (Wolf-Hirschhorn syndrome candidate 1), is involved in the regulation of apoptosis, cell cycle and cell adhesion. It is involved in the dimethylation of H3K36, and MMSETII is involved in the histone methylation of H3K4, H3K27, H3K36 and H4K20. This functionality of this protein is mediated through its SET (su(var)3-9, enhancer-of-zeste and trithorax) domain. Chromosomal translocation t(4;14) of this gene is implicated in multiple myeloma (MM), and correlates with poor prognosis.[1] In gastric cancer, the mRNA levels of WHSC1 are related to the response to first-line FOLFOX (folinic acid, fluorouracil (5-FU), oxaliplatin) and second-line docetaxel therapy. Therefore, this gene can be used to determine chemotherapy in gastric cancer cases.[4]

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.