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SRP0129

Sigma-Aldrich

Dot1L human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):

DOT1-like, histone H3 methyltransferase, H3-K79-HMTase, KIAA1814, KMT4, Lysine N-methyltransferase 4

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50 μG
$713.00

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50 μG
$713.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

$713.00


Usually ships in 2 weeks.

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

73 kDa

packaging

pkg of 50 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... DOT1L(84444)

General description

Human DOT1L, GenBank Accession No. NM_032482), amino acids 2-416 with N-terminal ST tag, MW = 73 kDa, expressed in E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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