SAB4200247
Anti-GP1BA antibody, Mouse monoclonal
clone PM6/40, purified from hybridoma cell culture
Synonym(s):
MonoclonalAnti-BSS, MonoclonalAnti-CD42B, MonoclonalAnti-CD42balpha, MonoclonalAnti-GP1B, MonoclonalAnti-MGC34595, MonoclonalAnti-glycoprotein Ib (platelet), alpha polypeptide
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PM6/40, monoclonal
form
buffered aqueous solution
mol wt
antigen ~140 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
flow cytometry: 30-60 μg/mL using human platelet
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GP1BA(2811)
General description
Monoclonal Anti-GP1BA (mouse IgG1 isotype) is derived from the hybridoma PM6/40 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. GP1BA, also known as glycoprotein Ib (GP Ib) and CD42b, is a platelet surface membrane glycoprotein. GP Ib is a heterodimer, composed of an α and β chain, that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the α- and β-subunits with platelet glycoprotein IX and platelet glycoprotein V. The glycoprotein Ib platelet subunit α (GP1BA) gene is mapped to human chromosome 17p13.2.
Specificity
Monoclonal Anti-GP1BA recognizes human GP1BA/CD42b.
Immunogen
human platelet membranes.
Application
Anti-GP1BA antibody, Mouse monoclonal is suitable for:
- immunoblotting
- fluorescence-activated cell sorting (FACS)
- immunohistochemistry
Biochem/physiol Actions
Glycoprotein Ib (GP Ib) functions as a receptor for von Willebrand factor (VWF). It regulates the adhesion of blood platelets to damaged blood vessel walls and the subsequent platelet aggregation. It also transmits signals leading to platelet activation, aggregation and secretion. The binding of the GP Ib-IX-V complex to VWF, facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. The interaction between Gp Ib and VWF was found to induce apoptotic events in platelets, suggesting a mechanism for platelet clearance or some thrombocytopenic disease. Furthermore, several polymorphisms and mutations have been described in this gene, some of which are the cause of Bernard Soulier syndromes and platelet-type von Willebrand disease.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Alan T Nurden et al.
British journal of haematology, 165(2), 165-178 (2013-11-30)
Genetic defects of platelets constitute rare diseases that include bleeding syndromes of autosomal dominant, recessive or X-linked inheritance. They affect platelet production, resulting in a low circulating platelet count and changes in platelet morphology, platelet function, or a combination of
Platelets
Encyclopedia of Immunology, 1973-1976 null
Ilaria Canobbio et al.
Cellular signalling, 16(12), 1329-1344 (2004-09-24)
The glycoprotein Ib-V-IX is one of the major adhesive receptors expressed on the surface of circulating platelets. It is composed of four different polypeptides-GPIbalpha, GPIbbeta, GPIX, and GPV-and represents a multifunctional receptor able to interact with a number of ligands
Shi-Zhong Luo et al.
Journal of molecular biology, 382(2), 448-457 (2008-08-05)
As the receptor on the platelet surface for von Willebrand factor, glycoprotein (GP) Ib-IX complex is critically involved in hemostasis and thrombosis. How the complex is assembled from GP Ibalpha, GP Ib beta and GP IX subunits, all of which
Jose A Guerrero et al.
Blood, 114(27), 5541-5546 (2009-10-08)
Platelet-type von Willebrand disease (PT-VWD) is a bleeding disorder of the platelet glycoprotein Ib-IX/von Willebrand factor (VWF) axis caused by mutations in the glycoprotein Ib-IX receptor that lead to an increased affinity with VWF. In this report, platelets from a
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