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Key Documents

S4576

Sigma-Aldrich

CleanCut Protease from Staphylococcus aureus

500-1000 units/mg protein

Synonym(s):

SplB Serine Protease

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Staphylococcus aureus

assay

95% (SDS-PAGE)

form

lyophilized powder

specific activity

500-1000 units/mg protein

Storage temp.

−20°C

Application

CleanCut Protease can be used to cleave fusion proteins with the specific sequence LVPWELQ↓-E(1).
CleanCut Protease has been used in a study to assess enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation. [1] Clean cut protease has also been used in a study to investigate immobilized residue-specific endoproteinases for protein sequencing. [2]

Unit Definition

One unit will cleave >90% of 100 μg of cleavage control protein in 100 mM Tris-HCl, pH 8.0, in a total volume of 100 μL at 20 °C in 16 hours.

Physical form

Supplied as a lyophilized powder containing NaCl, KCl, and sodium and potassium phosphate

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
050M1416

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Biochemical and structural characterization of SpID protease from Staphylococcus aureus
Zdzalik M. et al.
PLoS ONE, 8(10), e76812-e76812 (2013)
Immobilized residue-specific endoproteinases for protein sequencing
Ronnenberg, J., et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 656, 169-177 (1994)
Enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation
Arguello, M.
Journal of Membrane Science , 216, 121-134 (2003)
P B Conrad et al.
Biotechnology and bioengineering, 57(6), 631-641 (1999-04-01)
Recovery of an aqueous bioconversion product from complex, two-phase Pseudomonas putida broths containing 20% (v/v) soybean oil presents a significant challenge for downstream processing. Although not used before in multiple-phase separation for complex biotech products, crossflow filtration employing ceramic filters

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