S1311
Anti-SIRPα1 (SHPS-1) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous glycerol solution
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
mol wt
antigen 100 kDa
antigen 130 kDa
antigen 50 kDa
antigen 65 kDa
species reactivity
mouse, rat
technique(s)
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 4 μg/mL using RIPA lysates of mouse M1 myeloblast
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Sirpa(19261)
General description
Signal regulatory proteins (SIRPs) are a class of transmembrane glycoproteins with extracellular domains resembling the immunoglobulins. The SIRP family consists of three members, SIRPα, SIRPβ and SIRPγ. In rat there are two SIRPβ members, SIRPβ1 and SIRPβ2. SIRP members are ubiquitously present on the surface of all immune system cells. SIRPα (SHP-1) is expressed on neurons, myeloid dendritic cells, mast cells, macrophages and granulocytes. The most studied role of SIRPα is the inhibition of host cell phagocytosis by the macrophages. SIRPα is reportedly involved in clearance of aged erythrocytes, regulation of production of TNF-α induced by LPS, controlling the migration of myeloid cells and maturation of dendritic cells
Anti-SIRPα1 (SHPS-1) recognizes the differentially glycosylated forms of SIRPα1(SHPS-1) of 130, 100 and 65 kDa. It also detects a single band at 50 kDa.
Anti-SIRPα1 (SHPS-1) recognizes the differentially glycosylated forms of SIRPα1(SHPS-1) of 130, 100 and 65 kDa. It also detects a single band at 50 kDa.
Specificity
The antibody recognizes SIRPα1 (signal-regulatory protein) also known as BIT, SHPS-1, or p84. By immunoblotting, the antibody recognizes the differentially glycosylated forms of SIRPα1 (130, 100 and 65 kDa). It also detects a single band at 50 kDa.
Immunogen
fusion protein corresponding to the cytoplasmic domain of rat SHPS-1 (residues 418-481).
Application
Anti-SIRPα1 may be used in immunoblotting application at a working concentration of 4 μg/mL using mouse myeloblast whole cell lysate. The antibody is suitable for protein microarray and immunoprecipitation.
Physical form
Solution in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
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Takuji Yamao et al.
The Journal of biological chemistry, 277(42), 39833-39839 (2002-08-09)
SHPS-1 is a receptor-type glycoprotein that binds and activates the protein-tyrosine phosphatases SHP-1 and SHP-2, and thereby negatively modulates intracellular signaling initiated by various cell surface receptors coupled to tyrosine kinases. SHPS-1 also regulates intercellular communication in the neural and
Ellen M van Beek et al.
Journal of immunology (Baltimore, Md. : 1950), 175(12), 7781-7787 (2005-12-13)
Signal regulatory proteins (SIRPs) constitute a family of transmembrane glycoproteins with extracellular Ig-like domains. Several SIRP family members have thus far been identified on myeloid and other cells in man, mouse, rat, and cattle. In the present study, we provide
S Adams et al.
Journal of immunology (Baltimore, Md. : 1950), 161(4), 1853-1859 (1998-08-26)
Signal-regulatory proteins (SIRP) are transmembrane glycoproteins with three extracellular Ig-like domains, closely related to Ag receptors Ig, TCR, and MHC, and a cytoplasmic domain with two immunoreceptor with tyrosine-based inhibition motifs that can interact with src homology 2 domain-containing phosphatases.
Tomomi Ishikawa-Sekigami et al.
Blood, 107(1), 341-348 (2005-09-06)
The lifespan of circulating red blood cells (RBCs) produced in bone marrow is determined by their elimination through phagocytosis by splenic macrophages. The mechanism by which RBC elimination is regulated has remained unclear, however. The surface glycoprotein SHPS-1, a member
Akiko Hayashi et al.
The Journal of biological chemistry, 279(28), 29450-29460 (2004-05-05)
SIRPbeta (signal-regulatory protein beta) is a transmembrane protein that is expressed in hematopoietic cells but whose functions are unknown. We have now cloned mouse SIRPbeta cDNA and have shown that the gene is expressed in various tissues in addition to
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