R3152
RAN human
>90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), buffered aqueous glycerol solution
Synonym(s):
Ras related nuclear protein, TC4
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About This Item
MDL number:
UNSPSC Code:
12352202
Specificity
Binds GDP at more than 0.1 molar ratio.
Application
Ran, N-terminal histidine tagged, is a 26.6 kDa protein. It appears on SDS-PAGE as a 31 kDa protein in low ionic strength buffer. In high ionic strength buffers, it appears as a ladder of bands probably representing oligomerization. Ran was purified in the presence of GDP.
Biochem/physiol Actions
Ran (TC4), a small GTP binding protein, is the heart of the nuclear transport process. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. In the nucleus, it exists in Ran-GTP form, which is maintained by the Ran-Guanine nucleotide exchange protein, RCC1, a nucleus specific, chromatin bound protein. Ran-GTP forms complexes with transport proteins that shuttle from the nucleus to the cytoplasm i.e. improtin β, CRM1/exportin1. In the cytoplasm, Ran intrinsic GTPase activity is stimulated by Ran-GAP causing the hydrolysis of GTP to GDP and disassembly of the resulted Ran-GDP from the transport protein. Ran-GDP reenters the nucleus by active transport as NTF2/Ran-GDP complex.
Physical form
Solution in 20 mM HEPES-KOH, 110 mM potassium acetate, 2 mM magnesium acetate, 0.5 mM EGTA, 2 mM DTT, and 5% glycerol, pH 7.3.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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M S Moore et al.
Nature, 365(6447), 661-663 (1993-10-14)
Two cytosolic fractions (A and B) from Xenopus oocytes are sufficient to support protein import into the nuclei of digitonin-permeabilized cells. Fraction A recognizes the nuclear localization sequence (NLS) and binds the import substrate to the nuclear envelope, whereas fraction
Transport of proteins and RNAs in and out of the nucleus.
S Nakielny et al.
Cell, 99(7), 677-690 (2000-01-05)
E Conti et al.
Current opinion in cell biology, 13(3), 310-319 (2001-05-10)
Nucleocytoplasmic transport occurs through nuclear pore complexes (NPCs) and is mediated by saturable transport receptors that shuttle between the nucleus and cytoplasm. Our understanding of the molecular interactions underlying this process has improved dramatically as a result of the elucidation
D Görlich et al.
Annual review of cell and developmental biology, 15, 607-660 (1999-12-28)
The compartmentation of eukaryotic cells requires all nuclear proteins to be imported from the cytoplasm, whereas, for example, transfer RNAs, messenger RNAs, and ribosomes are made in the nucleus and need to be exported to the cytoplasm. Nuclear import and
Maria Chatzifrangkeskou et al.
The EMBO journal, 38(16), e101168-e101168 (2019-08-16)
Nuclear actin participates in many essential cellular processes including gene transcription, chromatin remodelling and mRNA processing. Actin shuttles into and out the nucleus through the action of dedicated transport receptors importin-9 and exportin-6, but how this transport is regulated remains
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