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R2507

Sigma-Aldrich

Reactive Blue 4−Agarose

saline suspension

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About This Item

MDL number:
UNSPSC Code:
23151817
PubChem Substance ID:
NACRES:
NA.56

form

saline suspension

extent of labeling

2-7 mg per mL

matrix

cross-linked 4% beaded agarose

capacity

≥5 mg/mL binding capacity (human serum albumin)

storage temp.

2-8°C

SMILES string

O=C1C2=C(C(NC3=CC=C(S(=O)(O)=O)C(NC4=NC(Cl)=NC(Cl)=N4)=C3)=CC(S(=O)(O)=O)=C2N)C(C5=C1C=CC=C5)=O

InChI

1S/C23H14Cl2N6O8S2/c24-21-29-22(25)31-23(30-21)28-12-7-9(5-6-14(12)40(34,35)36)27-13-8-15(41(37,38)39)18(26)17-16(13)19(32)10-3-1-2-4-11(10)20(17)33/h1-8,27H,26H2,(H,34,35,36)(H,37,38,39)(H,28,29,30,31)

InChI key

RTLULCVBFCRQKI-UHFFFAOYSA-N

Gene Information

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Application

Reactive Blue 4-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Blue 4-agarose has been used to express human pituitary glutaminyl cyclase (hQC) in Drosophila S2 cells.

Physical form

Suspension in 0.5 M NaCl containing preservative

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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L Wang et al.
The Journal of biological chemistry, 275(14), 10154-10159 (2000-04-01)
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In glycosyltransferase-catalyzed reactions a new carbohydrate-carbohydrate bond is formed between a carbohydrate acceptor and the carbohydrate moiety of either a sugar nucleotide donor or lipid-linked saccharide donor. It is currently believed that most glycosyltransferase-catalyzed reactions occur via an electrophilic activation
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We purified DNase II from human liver to apparent homogeneity. The N-terminal amino acid sequences of each of three components constituting the purified mature enzyme were then separately determined by automatic Edman degradation. A combination of this chemical information and
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The SecYEG complex is a membrane-embedded channel that permits the passage of precursor proteins (preproteins) across the inner membrane of Escherichia coli. SecA is a molecular motor that associates with the SecYEG pore and drives the stepwise translocation of preproteins
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The M2-type pyruvate kinase was purified from human meningioma by ammonium sulfate precipitation, followed by ion exchange and affinity chromatography. The specific activity of the purified enzyme was 33.4 U/mg with a yield of 6.5%. The enzyme gave a single

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