Frozen tissue can be used with MAK312. In cases where direct sample processing is not feasible, it is advised to snap freeze tissue samples in liquid nitrogen and store them either in liquid nitrogen or at -80°C until further processing.
MAK312
NADP/NADPH Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.84
Recommended Products
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue.[1]
Features and Benefits
- Sensitive and accurate - Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96 well plate assay.
- Convenient - The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.
- High-throughput - Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.
Sensitive and accurate - Detection limit of 0.01 mM and linearity up to 1 mM NADP+ /NADPH in 96 well plate assay.
Convenient – The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.
High-throughput – Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.
Convenient – The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.
High-throughput – Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.
Suitability
Suitable for measuring NADP+ /NADPH concentrations and ratio in cell or tissue extracts.
Principle
Simple, direct and automation-ready procedures for measuring NADP+ /NADPH concentration are very desirable. This NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex = 530 nm/λem = 585 nm, is proportional to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+/ NADPH and with minimal interference (<1%) by NAD+ /NADH. This assay is a convenient, direct method to measure NADP+ /NADPH concentrations and ratio in cell or tissue extracts.
The NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lex = 530 nm/lem = 585 nm, is proportional to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ / NADPH and with minimal interference (<1%) by NAD+ /NADH. This assay is a convenient, direct method to measure NADP+ /NADPH concentrations and ratio in cell or tissue extracts.
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Danger
hcodes
Hazard Classifications
Met. Corr. 1 - Resp. Sens. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can the NAADP/NADPH assay kit MAK312 be used with frozen tissue?
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