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MAK113

Sigma-Aldrich

ATPase/GTPase Activity Assay Kit

sufficient for 200 colorimetric tests

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1 KIT
$509.00

$509.00

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1 KIT
$509.00

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

$509.00

Usually ships in 2 weeks.
Request a Bulk Order

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

2-8°C

Related Categories

General description

ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.

Principle

The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8B - Non-combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
113CE07A12
113CE02A21
113CE07A12
113CE02A21
113CD02A16

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Shannon E Hill et al.
PloS one, 12(7), e0180241-e0180241 (2017-07-26)
Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because
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Protein & cell, 11(2), 124-137 (2019-11-24)
Type VII secretion systems (T7SSs) are found in many disease related bacteria including Mycobacterium tuberculosis (Mtb). ESX-1 [early secreted antigen 6 kilodaltons (ESAT-6) system 1] is one of the five subtypes (ESX-1~5) of T7SSs in Mtb, where it delivers virulence
Yupeng Zhao et al.
iScience, 23(9), 101494-101494 (2020-09-12)
Atlastin (ATL) is a class of dynamin-like GTPases shaping endoplasmic reticulum (ER) by mediating homotypic membrane fusion. Defect of ATLs leads to abnormal ER structure and hereditary spastic paraplegia (HSP), a neurodegenerative disease with progressive spasticity. How ATLs are regulated
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Oncotarget, 7(13), 16282-16296 (2016-02-27)
Increased integrin β4 (ITGB4) level is accompanied by malignant progression of multiple carcinomas. However, selective therapeutic strategies against cancer cells expressing a high level of ITGB4 have not been reported. Here, for the first time, we report that a chiral
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Questions

1–6 of 6 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Does malachite green react with phosphate molecules in which one oxygen is substituted by sulfur when using GTPgammaS instead of GTP to detect RAN GTPase activity? Additionally, could malachite green turn blue in reaction with other molecules besides phosphate?

    1 answer

    1. Unfortunately, it has not been tested whether malachite green also reacts with phosphate molecules in which one oxygen is substituted with sulfur, and there is uncertainty about other molecules that could cause a blue color change. It is also possible that there might be contaminating free phosphate in the materials used for the assay. The datasheet for the kit contains instructions on how to check for any contaminating free phosphate.

      Helpful?

  4. Would storage of the MAK113 ATPase/GTPase Activity Assay Kit at -20°C for 4 days affect its performance?

    1 answer

    1. Based on available information, it is not expected that storage at -20°C would significantly affect the kit’s performance or shelf life.

      Helpful?

  5. Is the MAK113 ATPase/GTPase Activity Assay Kit compatible in both acidic and basic pH environments? Within what pH range is this assay compatible? I am attempting to measure the GTPase activity within a pH range of 5.5 - 8.0.

    1 answer

    1. As per the guidance, the assay has been optimized with Assay Buffer at pH 7.5. It's important to note that the assay is pH dependent, and the enzymatic reaction is stopped by adding an acidic reagent. With the samples themselves expected to be largely neutralized by the assay buffer, they should be suitable within the pH range of 5.5 to 8. However, it is not advisable to prepare a different buffer to test the reaction under more acidic conditions. There is a possibility that conducting the reaction under more acidic conditions, like pH 5.5, might inhibit enzyme activity.

      Helpful?

  6. What is the detailed composition of Malachite Green Reagent (MAK 113A) in the ATPase/GTPase Activity Assay Kit (MAK113)?

    1 answer

    1. A detailed composition of the reagent is not available as this is considered proprietary information.

      Helpful?

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