MAK288
Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.25
Recommended Products
detection method
fluorometric
relevant disease(s)
immunological diseases; neurological disorders; hematological disorder
storage temp.
−20°C
General description
Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP deficiency leads to severe combined immunodeficiency and also leads to hypouricemia and reduced urinary excretion of uric acid.[1]
Suitability
Suitable for the detection of Purine Nucleoside Phosphorylase activity in cell and tissue lysate or purified recombinant protein
Principle
In this Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at λex = 535nm/λem = 587nm. Limit of quantification is 0.005 μU of recombinant purine nucleoside phosphorylase.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
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Disorders of purine and pyrimidine metabolism.
Nyhan W L, et al.
Molecular Genetics and Metabolism, 86(1), 25-33 (2005)
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