MAK087
Acid Phosphatase Activity Fluorometric Assay Kit
sufficient for 500 fluorometric tests
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About This Item
EC Number:
UNSPSC Code:
12352200
Recommended Products
usage
sufficient for 500 fluorometric tests
detection method
fluorometric
storage temp.
−20°C
Gene Information
human ... ACP1(52) , ACP2(53)
mouse ... ACP1(11431) , ACP2(11432)
rat ... ACP1(24161) , ACP2(24162)
General description
The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.[1][2]
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of acid phosphatase in serum and other biological samples.
Principle
The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.
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Description
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Storage Class
12 - Non Combustible Liquids
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