M8407

Methotrexate hydrate

powder, suitable for cell culture, BioReagent

• Synonym(s): 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N¹⁰-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate
• Empirical Formula (Hill Notation): C₂₀H₂₂N₈O₅ · xH₂O
• CAS Number: 133073-73-1
• Molecular Weight: 454.44 (anhydrous basis)
• MDL number: MFCD00150847
• UNSPSC Code: 12352207
• PubChem Substance ID: 24897252
• NACRES: NA.75

Properties

• product name: Methotrexate hydrate, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)
• product line: BioReagent
• assay: ≥98% (HPLC)
• form: powder
• technique(s): cell culture | mammalian: suitable
• solubility: H₂O: insoluble
• antibiotic activity spectrum: neoplastics; parasites
• mode of action: enzyme | inhibits
• storage temp.: −20°C
• SMILES string: [H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
• InChI: 1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
• InChI key: FPJYMUQSRFJSEW-ZOWNYOTGSA-N
• Gene Information: human ... DHFR(1719)

Description

Application

Methotrexate hydrate has been used:

• as a dihydrofolate (DHFR) inhibitor in sphere forming assay culture
• as a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells
• in viability and co-culture assays

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

Biochem/physiol Actions

Methotrexate is a folic acid antagonist.

Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H301,H315,H319,H360FD
• pcodes: P202 - P264 - P280 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges