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L4021

Sigma-Aldrich

Prolactin human

recombinant, expressed in E. coli, lyophilized powder, BioReagent, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):

hPRL, LTH, Lactogenic Hormone, Luteotropic Hormone, PRL

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About This Item

CAS Number:
9002-62-4
MDL number:
MFCD00131553
UNSPSC Code:
51111800
NACRES:
NA.77

recombinant

expressed in E. coli

Quality Level

200

description

200 amino acid residue methionyl form of mature, recombinant human prolactin (amino acid residues 29-227).

product line

BioReagent

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.030-0.300 ng/mL EC50

mol wt

predicted mol wt ~24 kDa

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

water: 0.5000 mL, clear, colorless

UniProt accession no.

P01236

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... PRL(5617)

application

Use in cell culture applications to study prolactin regulation of cell signaling and gene expression.

Biochem/physiol Actions

Prolactin is a neuroendocrine hormone. The prolactin receptor is a transmembrane glycoprotein that belongs to the cytokine hematopoietic receptor family. A large number of cells and organs express the receptor, including B cells, T cells, macrophages, monocytes, and neutrophils. Prolactin signal transduction involves the JAK/STAT families and the src kinase family. Induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine.

Other Notes

Contains an N-terminal methionine not present in the natural product.

Physical form

Lyophilized from a solution in 10 mM phosphate and 50 mM sodium chloride, pH 8.0.

Reconstitution

It is recommended that sterile 4 mM HCl containing 1 mg/mL bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.

Analysis Note

The biological activity is assayed by proliferation of the rat lymphoma cell line, Nb2-11.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Extrapituitary prolactin: distribution, regulation, functions, and clinical aspects.
N Ben-Jonathan et al.
Endocrine reviews, 17(6), 639-669 (1996-12-01)
G Bellone et al.
Journal of cellular physiology, 163(2), 221-231 (1995-05-01)
The pituitary hormone prolactin (Prl) is known to act as a local regulator of immune cell function, and Prl-binding receptors (Prl-R) have been described to share distinctive features with the members of the newly described cytokine/hemopoietin receptor superfamily. Here we
Julien Auffret et al.
American journal of physiology. Endocrinology and metabolism, 305(10), E1309-E1318 (2013-09-26)
Prolactin (PRL) and placental lactogens stimulate β-cell replication and insulin production in pancreatic islets and insulinoma cells through binding to the PRL receptor (PRLR). However, the contribution of PRLR signaling to β-cell ontogeny and function in perinatal life and the
Bibiana Moreno-Carranza et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 305(7), R720-R726 (2013-08-21)
Prolactin (PRL) is a potent liver mitogen and proangiogenic hormone. Here, we used hyperprolactinemic rats and PRL receptor-null mice (PRLR(-/-)) to study the effect of PRL on liver growth and angiogenesis before and after partial hepatectomy (PH). Liver-to-body weight ratio
Gerda de Kuijper et al.
Research in developmental disabilities, 34(9), 2799-2809 (2013-06-25)
Individuals with intellectual disability frequently use antipsychotics for many years. This may have detrimental health effects, including neurological symptoms and metabolic and hormonal dysregulation, the latter possibly affecting bone metabolism. There is large variability in the degree in which antipsychotic
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