I4639
IgG4, Kappa from human myeloma plasma
purified immunoglobulin, >95% (SDS-PAGE)
Synonym(s):
Human IgG4-κ
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About This Item
Recommended Products
biological source
human
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
assay
>95% (SDS-PAGE)
shipped in
dry ice
storage temp.
−20°C
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.[1] IgG4, κ is obtained from human IgG4 κ myeloma sera and purified by fractionation, ion-exchange, and affinity chromatography.
Application
The purified IgG4, κ, may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunobinding, Western immunobloting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Purified IgG4, κ, was used as standard in ELISA[2][3] and for labelling human IgG subclasses by flow cytometry.[4]
Physical form
Solution in 0.02 M Tris buffered saline, pH 8.0.
Analysis Note
The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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