212407

Direct Blue 71

Dye content 50 %

• Empirical Formula (Hill Notation): C₄₀H₂₃N₇O₁₃S₄ · 4Na
• CAS Number: 4399-55-7
• Molecular Weight: 1029.87
• Colour Index Number: 34140
• EC Number: 224-531-4
• MDL number: MFCD00003971
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852733
• NACRES: NA.47

Properties

• form: powder
• composition: Dye content, 50%
• λmax: 594 nm
• ε (extinction coefficient): ≥15000 at 283-289 nm in water at 0.01 g/L; ≥30000 at 582-588 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].Nc1ccc2c(O)c(N=Nc3ccc(N=Nc4ccc(N=Nc5cc(c6cccc(c6c5)S([O-])(=O)=O)S([O-])(=O)=O)c7ccccc47)c8ccc(cc38)S([O-])(=O)=O)c(cc2c1)S([O-])(=O)=O
• InChI: 1S/C40H27N7O13S4.4Na/c41-22-8-10-25-21(16-22)17-38(64(58,59)60)39(40(25)48)47-46-35-15-14-34(28-11-9-24(20-30(28)35)61(49,50)51)45-44-33-13-12-32(26-4-1-2-5-27(26)33)43-42-23-18-31-29(37(19-23)63(55,56)57)6-3-7-36(31)62(52,53)54;;;;/h1-20,48H,41H2,(H,49,50,51)(H,52,53,54)(H,55,56,57)(H,58,59,60);;;;/q;4*+1/p-4
• InChI key: QTTDXDAWQMDLOF-UHFFFAOYSA-J

Description

General description

Direct blue 71 is an azo dye with three azo bonds. It is useful to stain membrane-immobilized antibodies and proteins for the investigation of antigen-antibody interactions.

Application

Direct blue 71 has been used to stain sliced tissue sections mounted on the PVDF for in situ proteome analysis, and to stain the electroblotted membranes and sections of the brain to visualize the structure. Direct blue staining is also suitable for the assessment of protein binding and qualitative validation of protein concentration.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable