I2158
Anti-phospho-Insulin Receptor/Insulin-Like Growth Factor-1 Receptor (pTyr1162/1163) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
MDL number:
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
human, rat
technique(s)
immunocytochemistry: suitable
western blot: 1:1000 using CHO T-cells transfected with a vector containing insulin receptor and stimulated with insulin.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IGF1R(3480)
rat ... Igf1r(25718)
General description
Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. IGF 1R is fully activated by phosphorylation of tyrosine residues at Tyr1162 and Tyr1163. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1R signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate
Anti-phospho-IR/IGF1R (pTyr1162/1163) specifically recognizes human and rat insulin receptor and insulin-like growth factor-1 receptor phosphorylated at tyrosines 1162 and 1163.
Anti-phospho-IR/IGF1R (pTyr1162/1163) specifically recognizes human and rat insulin receptor and insulin-like growth factor-1 receptor phosphorylated at tyrosines 1162 and 1163.
Immunogen
synthetic phosphopeptide derived from the region of IR that contains tyrosines 1162 and 1163.
Application
Anti-phospho-IR/IGF1R (pTyr1162/1163) antibody may be used for immunoblotting at a working antibody dilution of 1:1000. The antibody is suitable for immunocytochemistry.
Target description
The catalytic loops within the tyrosine kinase domains of the IR/IGF1R contain a three tyrosine motif corresponding to Tyr1158, 1162, and 1163 (for IR) and Tyr1131, 1135, and 1136 (for IGF1R). Autophosphorylation within the activation loop proceeds in a processive manner.
Physical form
Anti-phospho-IR/IGF1R (pTyr1162/1163) is supplied as a solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Lysophosphatidylcholine inhibits insulin-induced akt activation through protein kinase C-α in vascular smooth muscle cells
Motley ED et al
Hypertension, 3, 508-512 (2002)
H Yu et al.
Journal of the National Cancer Institute, 91(2), 151-156 (1999-01-29)
Insulin-like growth factors (IGFs), in particular IGF-I and IGF-II, strongly stimulate the proliferation of a variety of cancer cells, including those from lung cancer. To examine the possible causal role of IGFs in lung cancer development, we compared plasma levels
J M Chan et al.
Science (New York, N.Y.), 279(5350), 563-566 (1998-02-07)
Insulin-like growth factor-I (IGF-I) is a mitogen for prostate epithelial cells. To investigate associations between plasma IGF levels and prostate cancer risk, a nested case-control study within the Physicians' Health Study was conducted on prospectively collected plasma from 152 cases
S E Hankinson et al.
Lancet (London, England), 351(9113), 1393-1396 (1998-05-21)
Insulin-like growth factor (IGF)-I, a mitogenic and antiapoptotic peptide, can affect the proliferation of breast epithelial cells, and is thought to have a role in breast cancer. We hypothesised that high circulating IGF-I concentrations would be associated with an increased
O Manousos et al.
International journal of cancer, 83(1), 15-17 (1999-08-17)
Recent data suggest that the IGF system plays an important role in the pathogenesis of several forms of human cancer, and there is evidence that IGFs acting in an autocrine and paracrine manner may also affect colorectal cancer risk. We
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
