No, if using a 95% ethanol step before eosin staining, it is important to avoid dragging water into the alcoholic eosin solution. Introducing too much water might make the solution more alkaline, which can affect the staining. pH is not typically measured as it tends to stain the pH electrode. Additionally, because this is a colored solution, using pH papers is not a viable option. If staining is poor, the solution either needs to be replaced, or it is possible to add 0.5 mL of glacial acetic acid per 100 ml of eosin Y solution to improve the staining.
Recommended Products
form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
concentration
0.5 % (w/v) in acidified ethanol
application(s)
hematology
histology
storage temp.
room temp
Looking for similar products? Visit Product Comparison Guide
Application
General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
Other Notes
Certified Eosin Y, 0.5% (w/v) in acidified 90% ethanol
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Sens. 1
Storage Class
3 - Flammable liquids
wgk_germany
WGK 2
flash_point_f
55.4 °F - closed cup
flash_point_c
13 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Wei-Peng Li et al.
ACS nano, 11(6), 5826-5835 (2017-05-19)
Traditional wound care methods include wound infection control, adequate nutritional supplements, education of changing position every 2-3 h to avoid tissue hypoxia, vacuum assistant closure, debridement, skin graft, and tissue flap. Electric current stimulation, ultrasound, laser, and hydrotherapy have emerged
David J Forsthoefel et al.
eLife, 9 (2020-04-03)
Proper function and repair of the digestive system are vital to most animals. Deciphering the mechanisms involved in these processes requires an atlas of gene expression and cell types. Here, we applied laser-capture microdissection (LCM) and RNA-seq to characterize the
Kavaljit H Chhabra et al.
Molecular metabolism, 6(10), 1274-1285 (2017-10-17)
Hypothalamic arcuate nucleus-specific pro-opiomelanocortin deficient (ArcPomc We measured RSNA by multifiber recording directly from the nerves innervating the kidneys in ArcPomc Consistent with our hypothesis, we found that basal RSNA was decreased in ArcPomc Hence, we conclude that ArcPomc is
Wenjiao Li et al.
Cell death and differentiation, 26(8), 1379-1395 (2018-10-14)
RASSF1A (Ras association domain family 1 isoform A) is a tumor suppressor and frequently inactivated by promoter hypermethylation in hepatocellular carcinoma (HCC). Autophagy is to degrade misfolded or aggregated proteins and dysfunctional organelles. Autophagy defects enhance oxidative stress and genome
Jing Liu et al.
Nature communications, 11(1), 6396-6396 (2020-12-18)
Clinical studies reveal changes in blood eosinophil counts and eosinophil cationic proteins that may serve as risk factors for human coronary heart diseases. Here we report an increase of blood or heart eosinophil counts in humans and mice after myocardial
-
Does the Eosin Y alcoholic solution (HT1101) need to be pH tested regularly?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
