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H0290

Sigma-Aldrich

Hartman′s Fixative

histological tissue fixative

Synonym(s):

Davidson′s Fixative

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

form

liquid

application(s)

hematology
histology

storage temp.

room temp

Related Categories

General description

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination.

Application

An overnight fixative used for the visualization of lymph nodes in radical dissection specimens. It is helpful in visualizing both both breast and colon specimens by turning lymph nodes white. Moore and Barr used a version of Hartman′s fixative for fixing skin biopsy specimens for study of fine nuclear details. Rat testes for immunohistochemical and morphological comparison were fixed using conventional and modified Hartman′s fixative for 24 hours, while eyes and testes for other analyses were fixed for 48 hours.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

75.2 °F

flash_point_c

24 °C

Certificates of Analysis (COA)

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Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid.
Latendresse J R, et al.
Toxicologic Pathology, 30(4), 524-533 (2002)
John R Latendresse et al.
Toxicologic pathology, 30(4), 524-533 (2002-08-22)
Most recent revisions of regulatory guidelines for testing effects of chemicals on reproduction recommend Bouin's fluid (BF) or a "comparable fixative" instead of formalin to preserve the morphologic detail of testes for histopathological evaluation. However, picric acid in BF is
Dan Wang et al.
Biology of reproduction, 102(5), 1033-1044 (2020-02-11)
Fertilization is one of the fundamental biological processes, but so far, we still do not have a full understanding of the underlying molecular mechanism. We have identified a human acrosome protein, LY6/PLAUR domain containing 4 (LYPD4), expressed specifically in human
Generation and functional characterization of a conditional Pumilio2 null allele.
Lin K, et al.
Journal of biomedical research (2017)
G J Nuovo et al.
The American journal of pathology, 134(4), 837-842 (1989-04-01)
In situ hybridization is used commonly for detection of human papillomavirus (HPV) DNA. There is little information, however, on whether the detection of HPV DNA by in situ hybridization can be affected by the way in which the tissue is
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