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GE29-0915-97

Sigma-Aldrich

Superose® 6 Increase 5/150 GL

Cytiva 29-0915-97, 8.6 μm avg. part. size, 5/150 GL

Synonym(s):

Superose® 6 Increase

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superose®

mol wt

5000-5000000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 29-0915-97

parameter

<0.15 mL/min flow rate
<0.35 mL/min flow rate
<0.75 mL/min flow rate
3 Mpa (435 psi)
4-50 μL sample volume

bed volume

24 mL

matrix

cross-linked agarose
polymeric

matrix active group

agarose phase

avg. part. size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

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General description

Superose® 6 Increase 5/150 GL is a versatile, prepacked column for rapid purity check and screening by size exclusion chromatography for proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When time is limited and small buffer and sample consumption is important, this is the column of choice.

Features and Benefits

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Storage Class

3 - Flammable liquids

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Certificates of Analysis (COA)

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Articles

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Protocols

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Related Content

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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