GE28-9356-04

HiPrep^™ 16/60 Sephacryl^® S-400 HR

Cytiva 28-9356-04, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

• Synonym(s): HiPrep Chromatography Column, Sephacryl S-400 HR
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• material: polypropylene (PP)
• mol wt: 20000-8000000 Da (globular proteins)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9356-04
• parameter: <0.5 mL/min flow rate; <5 mL sample volume; 0.15 Mpa (22 psi)
• bed volume: 120 mL
• column L × I.D.: 60 cm × 16 mm
• matrix: dextran-acrylamide particle platform; allyl dextran and N, N’-methylenebisacrylamide; polymeric
• matrix active group: dextran-acrylamide phase
• avg. part. size: 50 μm
• cleaning: 2-13
• working range: 3-11
• separation technique: size exclusion (SEC)

Description

General description

HiPrep^™ 16/60 Sephacryl^® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.

Application

Sephacryl^® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.

Features and Benefits

• Gel filtration columns for low to medium pressure AKTA systems.
• The HiPrep^™ Sephacryl^® series offer reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep^™ Sephacryl^® columns speed up protein purification.
• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

gel filtration columns for low to medium pressure ÄKTA systems+AE14

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva

Sephacryl is a registered trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids