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FLAAB

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer

lyophilized powder

Synonym(s):

ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP

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$90.94
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$526.00

$90.94

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1 VIAL
$90.94
5 VIALS
$301.00
10 VIALS
$526.00

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.84

$90.94

List Price$98.10Save 7%
Web-Only Promotion

Available to ship onApril 29, 2025Details

form

lyophilized powder

Quality Level

200

technique(s)

activity assay: suitable

storage temp.

−20°C

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General description

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer is a component of the ATP bioluminescent assay kit that may be employed for the quantitative bioluminescent determination of ATP in experimental samples.

Application

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer has been used to determine the level of adenosine kinase activity in isolated sections of mouse hippocampi[1]. It has also been used to measure the ATP content in oocytes[1] and harderian gland(HG) tissue homogenates.[2]

Preparation Note

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer contains MgSO4, dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA, bovine serum albumin (BSA), and tricine buffer salts.

Reconstitution

Reconstitute with 50 mL of sterile water.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ6656
SLCR1286
0000294088
0000261819
0000261818

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Andrea Boyd-Tressler et al.
The Journal of biological chemistry, 289(39), 27246-27263 (2014-08-13)
Anti-tumor immune responses have been linked to the regulated release of ATP from apoptotic cancer cells to engage P2 purinergic receptor signaling cascades in nearby leukocytes. We used the Jurkat T cell acute lymphocytic leukemia model to characterize the role
Peter Stief et al.
BMC microbiology, 14, 35-35 (2014-02-13)
A wealth of microbial eukaryotes is adapted to life in oxygen-deficient marine environments. Evidence is accumulating that some of these eukaryotes survive anoxia by employing dissimilatory nitrate reduction, a strategy that otherwise is widespread in prokaryotes. Here, we report on
Nicolette Gouder et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(3), 692-701 (2004-01-23)
Endogenous adenosine in the brain is thought to prevent the development and spread of seizures via a tonic anticonvulsant effect. Brain levels of adenosine are primarily regulated by the activity of adenosine kinase. To establish a link between adenosine kinase
Hana M Russo et al.
Journal of immunology (Baltimore, Md. : 1950), 197(4), 1353-1367 (2016-07-08)
Canonical inflammasome activation induces a caspase-1/gasdermin D (Gsdmd)-dependent lytic cell death called pyroptosis that promotes antimicrobial host defense but may contribute to sepsis. The nature of the caspase-1-dependent change in plasma membrane (PM) permeability during pyroptotic progression remains incompletely defined.
Nobuhiro Moro et al.
Experimental and therapeutic medicine, 21(6), 575-575 (2021-04-15)
The aim of the current study was to determine effects of mild traumatic brain injury (TBI), with or without blockade of purinergic ATP Y1 (P2Y1) receptors or store-operated calcium channels, on extracellular levels of ATP, glutamate, glucose and lactate. Concentrations
View All Related Papers

Questions

  1. It seems that you are currently trying to perform the ATP assay (Sigma Cat# FLAA) on cardiac tissue. The kit appears to be working, as your standard curve is functioning, but you are obtaining very low numbers for your samples, significantly below your standard curve. Given that the heart should contain an abundance of ATP, you are concerned that your extraction protocol might be the issue. You are wondering if there is an extraction protocol available for this tissue for the mentioned kit.

    1 answer

    1. The low value observed in the sample may be due to several factors. Since the standard curve is working well, it is likely that the lysis buffer and/or homogenization condition used is not optimized for assay reading. To verify the possible presence of inhibiting substances, one could add a known amount of ATP from a standard solution to the homogenate to see if the reading increases accordingly. It's possible that the lysis buffer used was not optimal to yield an adequate amount of ATP for detection. Currently, there is no recommended cardiac tissue preparation protocol for use with this kit. As the kit measures only soluble ATP, it is highly recommended to clarify the homogenized extract by centrifugation to obtain a supernatant solution for use with this assay.

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