F6131
Fetuin from fetal bovine serum
γ-irradiated, BioXtra, suitable for cell culture
Synonym(s):
Fetuin from fetal calf serum
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About This Item
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sterility
γ-irradiated
product line
BioXtra
form
powder
mol wt
48.4 kDa
technique(s)
cell culture | mammalian: suitable
solubility
water: 1 mg/mL, clear to slightly hazy, colorless to faintly yellow
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... FETUB(504615)
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General description
Fetuin is a protein component of serum which contributes to the attachment and spreading of cells in culture medium. Researchers often add serum for this purpose. Fetuin has been used at a concentration of 500 μg/ml to supplement serum-free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells. Fetuin can be used as a glycoprotein standard for carbohydrate structure in a glycoprotein. The structural elucidation of the carbohydrate portion of fetuin has been published. The cDNA and amino acid sequences has been reported. Fetuin contains 12 Cys residues, all involved in disulfide bridges.
The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%.1 This product usually assays at 5-8% total sialic acid. Nearly all of this sialic acid is bound. The amount of free sialic acid is typically less than 0.3%. A method for determining free sialic acid content has been published.
The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%.1 This product usually assays at 5-8% total sialic acid. Nearly all of this sialic acid is bound. The amount of free sialic acid is typically less than 0.3%. A method for determining free sialic acid content has been published.
Analysis Note
This product is cell culture tested (0.5 g/L) and is appropriate for use in cell culture applications.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Ultracentrifugal and electrophoretic studies on fetuin.
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