This product may be stored at room temperature. Stability studies on the product in solution have not been completed. However, the literature suggests that solutions are fairly stable. See the link below to a publication citing solution stability:
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/calcofluor-white.
F3543
Fluorescent Brightener 28
used as a stain and brightening agent
Synonym(s):
Calcofluor White M2R, Tinopal UNPA-GX
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Empirical Formula (Hill Notation):
C40H44N12O10S2
CAS Number:
Molecular Weight:
916.98
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
form
powder
mp
300 °C
εmax
40-60 at 238-242 nm in water at 0.01 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
OCCN(CCO)c1nc(Nc2ccccc2)nc(Nc3ccc(\C=C\c4ccc(Nc5nc(Nc6ccccc6)nc(n5)N(CCO)CCO)cc4S(O)(=O)=O)c(c3)S(O)(=O)=O)n1
InChI
1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+
InChI key
CNGYZEMWVAWWOB-VAWYXSNFSA-N
General description
Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps. It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.
Application
It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.[3]
signalword
Warning
hcodes
pcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The multifunctional beta-oxidation enzyme is required for full symptom development by the biotrophic maize pathogen Ustilago maydis.
Klose J and Kronstad JW
Eukaryotic Cell, 5, 2047-2047 (2006)
Alok Arun et al.
The New phytologist, 197(2), 503-510 (2012-10-31)
The model brown alga Ectocarpus has a haploid-diploid life cycle, involving alternation between two independent multicellular generations, the gametophyte and the sporophyte. Recent work has shown that alternation of generations is not determined by ploidy but is rather under genetic
Christine Seah et al.
Medical mycology, 50(3), 324-327 (2011-08-24)
We compared the FXG™: RESP (Asp +) real-time PCR assay (Myconostica Ltd) with two microscopic staining methods (direct immunofluorescence [IFA] and calcofluor white) for the detection of Pneumocystis jirovecii in 411 respiratory specimens submitted for P. jirovecii examination. We considered
Luis Vidali et al.
PloS one, 4(5), e5744-e5744 (2009-05-30)
Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.
Candida albicans biofilm-defective mutants.
Richard ML
Eukaryotic Cell, 4, 1493-1493 (2005)
-
What is the storage temperature of fluorescent brightener 28?
1 answer-
Helpful?
-
-
What are the excitation and emission wavelengths for Product F3543, Fluorescent Brightner 28?
1 answer-
Excitation is at 365 nm and emission at is at 435 nm.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Is there a reference for use of Product F3543, Fluorescent Brightner 28?
1 answer-
3 different staining applications are found in the following reference: Clark, G; Staining Procedures, 4th Edition, Williams & Wilkins, Baltimore 1981 pages 46-48. This product is used to monitor removal or regeneration of plant cell walls. A protocol for using this product with plant protoplasts is described. D.W. Galbraith, Physiologia Plantarum, 53, 111-116 (1981).
Helpful?
-
-
How is Product F3543, Fluorescent Brightner 28, dissolved for use?
1 answer-
The product can be diluted in DMSO at a concentration of 50 mg/ml. The product can be dissolved in water at a concentration of 1 mg/ml, although the use of 50% sodium hydroxide might be required to fully solubilize the product.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
