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F3543

Sigma-Aldrich

Fluorescent Brightener 28

used as a stain and brightening agent

Synonym(s):

Calcofluor White M2R, Tinopal UNPA-GX

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About This Item

Empirical Formula (Hill Notation):
C40H44N12O10S2
CAS Number:
4404-43-7
Molecular Weight:
916.98
EC Number:
224-548-7
MDL number:
MFCD00308955
UNSPSC Code:
12171500
PubChem Substance ID:
24894849
NACRES:
NA.47

form

powder

mp

300 °C

εmax

40-60 at 238-242 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OCCN(CCO)c1nc(Nc2ccccc2)nc(Nc3ccc(\C=C\c4ccc(Nc5nc(Nc6ccccc6)nc(n5)N(CCO)CCO)cc4S(O)(=O)=O)c(c3)S(O)(=O)=O)n1

InChI

1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+

InChI key

CNGYZEMWVAWWOB-VAWYXSNFSA-N

General description

Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps. It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.
Fluorescent Brightener 28 has been used for the staining of fungal cell walls. It has also been used for the staining of Candida albicans biofilm.

Application

It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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