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Sigma-Aldrich

CelLytic™ Y Plus Kit

For enzymatic yeast cell lysis

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.56

shipped in

dry ice

storage temp.

−20°C

Application

The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immuno­reactivity and biological activity.
The cell extract is compatible with:
  • Reporter gene expression assays (e.g., β-galactosidase, alkaline-phosphatase)
  • Immunoassays (Western blots, immunoprecipitation)
  • Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins)
  • DNA-protein interaction assay (e.g., gel-shift)
  • Coomassie and silver staining
The kit is suitable for spheroplast formation and protein extraction from 10-40 grams of yeast cell pellet.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Reaction Buffer 120 mL
  • 1 M DTT 5 mL
  • Lyticase 25000 U
  • Extraction buffer 60 mL
  • Triton™ X-100 10 mL
  • Protease inhibitor cocktail 2 mL

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F

flash_point_c

87 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
127M4167V
11171016
047M4115V
11170406
075M4053V

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Articles

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

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