CS0730
Tyrosine Kinase Assay Kit
sufficient for 50 reactions
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About This Item
UNSPSC Code:
12352200
Recommended Products
usage
sufficient for 50 reactions
shipped in
dry ice
storage temp.
−20°C
General description
The assay is based on immunoprecipitation of the tyrosine kinase of interest using the specific anti-tyrosine kinase antibody. The immunoprecipitated kinase is incubated with its substrate (poly [Glu, Tyr] 4:1) in the presence of (γ32P)-ATP. The incorporation of the radioactive-phosphate is measured.
Biochem/physiol Actions
Protein tyrosine kinases (PTKs) are involved in regulation of cell to cell signaling concerning growth, differentiation, adhesion, motility, and death. They play significant roles in the development of many disease states, including diabetes and cancer.
Analysis Note
The kit provides a peroxidase conjugated anti-phosphotyrosine antibody, which enables substrate analysis by dot blot. This assay serves as an alternative, short and easy non-radioactive detection method.
Kit Components Only
Product No.
Description
- Monoclonal Anti-Phosphotyrosine-Peroxidase antibody produced in mouse 1 vial
- Wash Buffer 10X 8 mL
- Assay Buffer For Tyrosine Kinase Activity 1 mL
- EZviewTM Red Protein A Affinity Gel 1 mL
- Epidermal Growth Factor Receptor 500 U
- P81 Cellulose Phosphate Squares 10 ea
- Mouse Monoclonal Anti-EGFR Antibody .05 mL
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