AFRC IAH-CC62
99011334, Reactivity: Anti-CD26 (formerly WC10), bovine
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About This Item
UNSPSC Code:
41106514
biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS-1
Reactivity
Anti-CD26 (formerly WC10), bovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The hybridoma IAH-CC62 secretes antibodies reactive with bovine CD26 - this was formerly known as WC10. This non-lineage specific antigen is present on bovine leukocytes and has a MW of 115 kDa and 39kDa. Cells have been grown on mouse macrophage feeder cells using hypoxanthine and thymidine at concentractions appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Immunogen
Bovine thymocytes
Application
Immunohistology, Flow cytometry, Immunoprecipation
Culture Medium
RPMI 1640 + 2 mM glutamine + HT supplement + 10% FBS
Subculture Routine
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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