Normal human testis. Cell line may have a finite life span, therefore passage number should be kept as low as possible. Population doublings have not been determined.
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. This cell line may have a finite life span therefore the population doubling time should be calculated at every passage to ensure cel
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