As indicated in the 'DESCRIPTION' section of the product page, under 'Cell Line Description', these cells should be handled under laboratory containment level 2.
HeLa Cell Line human
93021013, human cervix, Epithelial
Synonym(s):
HeLa Cells, HeLa-CCL2 Cells, Henrietta Lacks Cells
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93021013-DNA-5UG
$402.00
1 VIAL
$563.00
$402.00
Usually ships in 1 week. (Orders outside of Europe & Scandinavian countries, allow an additional week for delivery
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93021013-DNA-5UG
$402.00
1 VIAL
$563.00
About This Item
UNSPSC Code:
41106514
$402.00
Usually ships in 1 week. (Orders outside of Europe & Scandinavian countries, allow an additional week for delivery
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Product Name
HeLa Cell Line human, epitheloid cervix carcinoma
biological source
human cervix
growth mode
Adherent
karyotype
aneuploidy
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human cervix epitheloid carcinoma
Cell Line Description
Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.
Application
HeLa cell line has been used to study whether Zika virus (ZIKV) infection alters centrosome number and spindle positioning in neural progenitor cells.[1] It has also been used to evaluate the cytotoxicity of extracts, fruits and jams from Sorbus sps.[2]
Virus studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm2 using 0.25% Trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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Bonjour, y a-t-il un pré-requis de Biosafety Level pour pouvoir commander ces cellules? Merci
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Hey! Regarding 93021013-DNA-5UG: Is this the cell line (frozen culture) or really (isolated?) DNA? Thanks, hannes
1 answer-
93021013-DNA-5UG is DNA extracted from the cell line.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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Are my Cell Line cells mycoplasma tested?
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ECACC routinely tests all manufactured cell banks for mycoplasma.
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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How many Cell Line cells are in the vial?
1 answer-
Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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