A375-C5 Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
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biological source
human skin
description
Human malignant melanoma IL-1 insensitive
packaging
tube of 5 μg 97011320-DNA-5UG
pkg of vial of cells 97011320-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human malignant melanoma IL-1 insensitive
Cell Line Description
The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.
Application
IL-1 resistance studies, receptor studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% FBS
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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