NCI-H69VCR/R
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96042330, human lung, Not specified
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106514
eCl@ss:
32190102
Pricing and availability is not currently available.
Recommended Products
biological source
human lung
packaging
tube of 5 μg 96042330-DNA-5UG
pkg of vial of cells 96042330-1VL
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human small cell lung cancer, drug-resistant
Cell Line Description
NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (Sigma Catalogue number. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Application
Drug-resistance studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 2.0 μg/ml vincristine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37ºC. Resuscitate in drug-free medium, add after growth has been established.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
