CFPAC-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91112501, human pancreas (liver metastasis), Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: human pancreas (liver metastasis)
• packaging: tube of 5 μg 91112501-DNA-5UG; pkg of vial of cells 91112501-1VL
• growth mode: Adherent
• karyotype: Hyperdiploid, (65-75 chromosomes)
• morphology: Epithelial
• products: Not specified
• receptors: Cystic fibrosis trans-membrane regulator (CFTR)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian pancreatic adenocarcinoma

Cell Line Description

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

Application

Cystic fibrosis studies, genetic analysis

Culture Medium

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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