Derived from a primary osteosarcoma of a 9 year old Caucasian female. Cells exhibit the type B phenotype for G6PD and the karyotype is subtetraploid with chromosome markers.
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/0.02% EDTA; 5% CO2; 37°C.
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