Cri du Chat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human skin
• packaging: tube of 5 μg 90102533-DNA-5UG; pkg of vial of cells 90102533-1VL
• growth mode: Adherent
• karyotype: 2n = 46, diploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian skin deletion in chromosome 5

Cell Line Description

Derived from the skin of an adult female Caucasian with Cri du Chat syndrome. The cells have a deletion in chromosome 5 and have a finite lifespan; up to 35 population doublings expected.

Application

Chromosome studies

Culture Medium

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Cells should not be centrifuged.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.