ARH 77

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88121201, human blood, Lymphoblast

• UNSPSC Code: 41106514

Properties

• biological source: human blood
• packaging: tube of 5 μg 88121201-DNA-5UG; pkg of vial of cells 88121201-1VL
• growth mode: Suspension
• karyotype: Hypodiploid
• morphology: Lymphoblast
• products: Immunoglobulin G (IgG1) (kappa)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human plasma cell leukaemia

Cell Line Description

Derived from a 33 year old female with plasma cell leukaemia. The cells synthesise IgG1 (kappa-light chain) and are EBNA positive.Although the cells are reported to originate from a patient with a plasma cell leukaemia , it has now been shown that this is in fact an EBV transformed B lymphoblastoid cell line .

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 1-4x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.