CRFK (BVD Ag negative)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86093002, feline kidney, Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: feline kidney
• packaging: tube of 5 μg 86093002-DNA-5UG; pkg of vial of cells 86093002-1VL
• growth mode: Adherent
• karyotype: Hypodiploid, modal no 36
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Cat kidney

Cell Line Description

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

Application

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.[1][2]

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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