Hep2 (HeLa derivative) Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86030501, human cervix, Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: human cervix
• description: Human Black, cervix carcinoma
• packaging: tube of 5 μg 86030501-DNA-5UG; pkg of vial of cells 86030501-1VL
• growth mode: Adherent
• karyotype: Modal no. 14
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human cervix carcinoma

Cell Line Description

Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Application

Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.[1][2]

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. After thawing the first subculture may take 7-10 days.

Other Notes

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