C10
12022901, human colon, Epithelial
Synonym(s):
C 10, C-10
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About This Item
UNSPSC Code:
41106514
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Product Name
C10, from human colon, 12022901
biological source
human colon
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Storage temp.
−196°C
Cell Line Origin
Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage B
Cell Line Description
The C10 cell line was established from a 71-year old male patient with moderately well differentiated adenocarcinoma of the descending colon classified as Dukes′ stage B.
DNA Profile
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 10,13
THO1: 6,9.3
TPOX: 8,9
vWA: 14,15
Amelogenin: X,Y
CSF1PO: 11
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 10,13
THO1: 6,9.3
TPOX: 8,9
vWA: 14,15
Culture Medium
Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
C10 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100 g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
C10 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100 g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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