CM3.B6
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
human blood
packaging
tube of 5 μg 11060101-DNA-5UG
pkg of vial of cells 11060101-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Small rounded cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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Cell Line Origin
Human anti-hepatitis C virus IgG1 secreting B cell
Cell Line Description
Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific for non-structural protein NS3 (helicase domain). This antibody binds to a major conformational epitiope located at the carboxyl-terminal end of the NS3 region that is recognised by approximately 90% from HCV-infected patients. CM3.B6 can be used in immunochemistry, HCV protein purification and HCV typing. CM3.B6 and B12.F8 (Sigma product number 11060102) were derived from the same patient as confirmed by STR profiling.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16
Culture Medium
RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interlukin-6 (IL-6).
Subculture Routine
Maintain cultures between 3-9 x 105/ml. 5% CO2; 37 °C. Media change every two days. Saturation density 1.1 x 106/ml
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
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