MTSV1-7 CE1
10081202, human breast, Cuboidal morphology at confluence
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UNSPSC Code:
41106514
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Product Name
MTSV1-7 CE1, 10081202, from human breast
biological source
human breast
growth mode
Adherent
karyotype
Not specified
morphology
Cuboidal morphology at confluence
products
Formation of disorganised structures in collagen type I gels.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Breast cancer, morphogenesis, adhesion properties
Cell Line Description
MTSV1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 transfected with ERBB2 expression vector, enabling the over-expression of ERBB2 proto-oncogene. Cultures of MTSV1-7 were transfected with the pSV2-erbB2 construct together with the pSV2neo plasmid. (See also 1-7HB2 Sigma catalog number 10081201).
The MTSV1-7 CE1 cell line is an ideal tool for investigation of the role of the ERBB2 receptor proto-oncogene in breast cancer morphogenesis and adhesion. MTSV1-7 CE1 cells over-express the ERBB2 receptor, inducing a reduced ability to undergo morphogenesis in vitro, and reduced expression of surface adhesion molecules E-Cadherin and alpha-2 integrin. Inhibition of morphogenesis and transcription of adhesion molecules in human mammary epithelial cells can be affected by signals generated by the ERBB2 receptor, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.
The MTSV1-7 CE1 cell line is an ideal tool for investigation of the role of the ERBB2 receptor proto-oncogene in breast cancer morphogenesis and adhesion. MTSV1-7 CE1 cells over-express the ERBB2 receptor, inducing a reduced ability to undergo morphogenesis in vitro, and reduced expression of surface adhesion molecules E-Cadherin and alpha-2 integrin. Inhibition of morphogenesis and transcription of adhesion molecules in human mammary epithelial cells can be affected by signals generated by the ERBB2 receptor, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO<sub>2</sub>; 37 °C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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