BHK21-InVitrus
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
hamster kidney
packaging
tube of 5 μg 05062302-DNA-5UG
pkg of vial of cells 05062302-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Syrian kidney, serum-free
Cell Line Description
BHK21CL13-2P cell line (Sigma Catalogue number. 84111301) adapted to grow in the chemically defined protein- and peptide-free InVitrus™ medium.
Culture Medium
InVitrus™ medium + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use InVitrus medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.
Subculture Routine
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Legal Information
InVitrus is a trademark of Cell Culture Technologies
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