Seed at 5 x 10,000 cells/ml. Subculture when cells reach 500,000 - 1,000,000 cells/ml (usually after 3-4 days) 5% CO2, 37° C. Start in 20% FBS when recovering from frozen.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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